Preliminary Phytochemical Screening and Antibacterial Properties of Crude Stem Bark Extracts and Fractions of Parkia biglobosa (Jacq.)

Molecules

Emmanuel O. Abioye⁴  David A. Akinpelu⁴  Olayinka A. Aiyegoro³  Mobolaji F. Adegboye²  Matthew O. Oni¹ 

[1] Department of Biological Sciences, Oduduwa University, Ipetumodu 234, Nigeria; E-Mail:;Department of Microbiology, North West University, Mafikeng Campus, Mmabatho 2745, South Africa; E-Mail:;GI Microbiology and Biotechnology Unit, Agricultural Research Council, Animal Production Institute, Irene 0062, South Africa; E-Mail:;Department of Microbiology, Obafemi Awolowo University, Ile-Ife 234, Nigeria; E-Mails:

关键词: minimum inhibitory concentration (MIC);    stem bark extract;    fraction;    phytochemistry;    resistance;    infections;    bioactive;    antibacterial;    antibiotics;   

DOI  :  10.3390/molecules18078485

来源: mdpi

【 摘 要 】

A methanolic crude extract of Parkia biglobosa was prepared and later partitioned in succession with different solvents of increasing polarity ranging from n-hexane, chloroform and ethyl acetate to butanol. Phytochemical screening of the extract revealed the presence of alkaloids, tannins, saponins, flavonoids, steroids, glycoside and sugars. The inhibition zones exhibited by the extract against the tested bacteria ranged between 14 ± 0.00 mm (against Escherichia coli) and 28 ± 0.71 mm (against Pseudomonas aeruginosa). The MIC of the methanolic extract of P. biglobosa against isolates ranged between 0.63 mg/mL and 5 mg/mL, while the MIC values exhibited by the n-hexane and aqueous fractions ranged between 0.63 mg/mL and 10 mg/mL. Overall the extract and fractions of P. biglobosa used in this work were found to possess antimicrobial properties which compared favourably with those of streptomycin. These observations make this plant a potential source of bioactive compounds that can be used in management of bacterial infections. The use of this plant as herbal medicaments in African countries and the reports on the toxicity of the plant further show that the plant is non-toxic to humans.

【 授权许可】

CC BY   
© 2013 by the authors; licensee MDPI, Basel, Switzerland.