Development of a Monoclonal Antibody-Based Sandwich ELISA for Peanut Allergen Ara h 1 in Food

International Journal of Environmental Research and Public Health

Juan Peng²  Shanshan Song²  Liguang Xu²  Wei Ma²  Liqiang Liu²  Hua Kuang¹ 

[1] State Key Laboratory of Food Science & Technology, School of Food Science & Technology, Jiangnan University, Wuxi 214122, China;

关键词: peanut allergen;    Ara h 1;    monoclonal antibody;    sandwich ELISA;   

DOI  :  10.3390/ijerph10072897

来源: mdpi

【 摘 要 】

We have established a highly sensitive sandwich enzyme-linked immunosorbent assay (ELISA) based on two monoclonal antibodies (mAb) to measure the content of the major peanut allergen Ara h 1 in foods. Two mAbs were selected out of 12 murine hybridoma cells secreting Ara h 1-specific antibody. Using mAb 6 as the capture antibody and HRP-labelled mAb 4 as the detection antibody, the limit of detection (LOD) the assay was 0.34 ng/mL. Cross-reaction analysis showed that this method was strongly specific and had no cross-reactions with Ara h 2, pea protein or soy protein. Sample analysis showed that this ELISA was a useful tool to monitor peanut allergens in food products by measuring Ara h 1 content.

【 授权许可】

CC BY   
© 2013 by the authors; licensee MDPI, Basel, Switzerland.