Solid-, Solution-, and Gas-state NMR Monitoring of 13C-Cellulose Degradation in an Anaerobic Microbial Ecosystem

Molecules

Akira Yamazawa²  Tomohiro Iikura³  Amiu Shino¹  Yasuhiro Date³ 

[1] RIKEN Center for Sustainable Resource Science, 1-7-22 Suehirocho, Tsurumi-ku, Yokohama, Kanagawa 230-0045, Japan; E-Mail:;Research Planning and Management Group, Kajima Technical Research Institute, KAJIMA Corporation, 2-19-1 Tobitakyu, Chofu, Tokyo 182-0036, Japan; E-Mail:;Graduate School of Medical Life Science, Yokohama City University, 1-7-29 Suehirocho, Tsurumi-ku, Yokohama, Kanagawa 230-0045, Japan; E-Mails:

关键词: solid-state NMR;    solution-state NMR;    gas-state NMR;    anaerobic digestion;    bacterial cellulose;   

DOI  :  10.3390/molecules18089021

来源: mdpi

【 摘 要 】

Anaerobic digestion of biomacromolecules in various microbial ecosystems is influenced by the variations in types, qualities, and quantities of chemical components. Nuclear magnetic resonance (NMR) spectroscopy is a powerful tool for characterizing the degradation of solids to gases in anaerobic digestion processes. Here we describe a characterization strategy using NMR spectroscopy for targeting the input solid insoluble biomass, catabolized soluble metabolites, and produced gases. ¹³C-labeled cellulose produced by Gluconacetobacter xylinus was added as a substrate to stirred tank reactors and gradually degraded for 120 h. The time-course variations in structural heterogeneity of cellulose catabolism were determined using solid-state NMR, and soluble metabolites produced by cellulose degradation were monitored using solution-state NMR. In particular, cooperative changes between the solid NMR signal and ¹³C-¹³C/¹³C-¹²C isotopomers in the microbial degradation of ¹³C-cellulose were revealed by a correlation heat map. The triple phase NMR measurements demonstrated that cellulose was anaerobically degraded, fermented, and converted to methane gas from organic acids such as acetic acid and butyric acid.

【 授权许可】

CC BY   
© 2013 by the authors; licensee MDPI, Basel, Switzerland.