Lipopolysaccharide induces raft domain expansion in membrane composed of a phospholipid-cholesterol-sphingomyelin ternary system:

Innate Immunity

KaoruNomura¹ 

关键词: cell membrane;    molecular motion;    raft domain;    ReLPS;    solid-state NMR;   

DOI  :  10.1177/1753425910365944

学科分类：生物科学（综合）

来源: Sage Journals

【 摘 要 】

The molecular behavior and interaction of Re-type lipopolysaccharide (ReLPS) and phospholipids were investigated in two different types of model membrane systems, a pure phospholipid membrane consisting of 1,2-dielaidoyl-snglycero-3-phosphoethanolamine (DEPE) and a raft-forming membrane composed of equimolar DEPE, sphingomyelin (SM), and cholesterol (Chol) by solid-state NMR spectroscopy. A remarkable influence of ReLPS on the property of lipid bilayer was found by analyzing the 13C-NMR spectra. Namely, while both liquid-ordered (Lo) and liquid-disordered (Ld) phases co-exist in DEPE/SM/Chol, only the Lo phase is present in DEPE/SM/Chol/ReLPS. This clearly indicates that ReLPS induces expansion of the raft area in the raft-forming membrane. The 1H spin-lattice relaxation times in the rotating frame T 1ρH in the two different membranes, DEPE/ReLPS and DEPE/SM/Chol/ReLPS, indicate that the motion of DEPE is affected by the presence of ReLPS, Chol, and SM, and much faster than that of ReLPS in both membranes...

【 授权许可】

CC BY