Highly Efficient Biotransformation of Polydatin to Resveratrol by Snailase Hydrolysis Using Response Surface Methodology Optimization

Molecules

Zi Wang¹  Li-Chun Zhao²  Wei Li¹  Lian-Xue Zhang¹  Jing Zhang¹ 

[1] College of Chinese Medicinal Materials, Jilin Agricultural University, Changchun 130118, China;The Affiliated Ruikang Hospital, Guangxi University of Chinese Medicine, Nanning 530011, China

关键词: snailase hydrolysis;    polydatin;    resveratrol;    response surface methodology;   

DOI  :  10.3390/molecules18089717

来源: mdpi

【 摘 要 】

Resveratrol (RV), a dietary antioxidant polyphenolic compound found in grapes and red wine, exerts a wide variety of pharmacological activities. However, lower content in plants compared with polydatin (PD, the glucoside of RV) limits its application in the food and pharmaceutical industries. In this paper, we carried out efficient biotransformation of PD to RV with 100% conversion yield by snailase hydrolysis. Moreover, response surface methodology (RSM) was used to optimize the effects of the reaction temperature, enzyme load, and reaction time on the conversion process. Validation of the RSM model was verified by the good agreement between the experimental and the predicted RV yield values. The optimum preparation conditions were as follows: temperature of 62.0 °C, enzyme load of 6.6%, and reaction time of 96 min. The proposed method may be highly applicable for the enzymatic preparation of RV for medicinal purposes.

【 授权许可】

CC BY   
© 2013 by the authors; licensee MDPI, Basel, Switzerland.