MicroRNA-143 Downregulates Interleukin-13 Receptor Alpha1 in Human Mast Cells

International Journal of Molecular Sciences

Shaoqing Yu¹  Ruxin Zhang¹  Chunshen Zhu²  Jianqiu Cheng²  Hong Wang¹ 

[1] Department of Otolaryngology, Huadong Hospital, Fudan University, Shanghai 200040, China; E-Mails:;Department of Otolaryngology, Jinan General Hospital of PLA, Jinan 250031, Shandong, China; E-Mails:

关键词: microRNA-143;    IL-13Rα1;    mast cell;    lentiviral vector;    allergy;   

DOI  :  10.3390/ijms140816958

来源: mdpi

【 摘 要 】

MicroRNA-143 (miR-143) was found to be downregulated in allergic rhinitis, and bioinformatics analysis predicted that IL-13Rα1 was a target gene of miR-143. To understand the molecular mechanisms of miR-143 involved in the pathogenesis of allergic inflammation, recombinant miR-143 plasmid vectors were constructed, and human mast cell-1(HMC-1) cells which play a central role in the allergic response were used for study. The plasmids were transfected into HMC-1 cells using a lentiviral vector. Expression of IL-13Rα1 mRNA was then detected by reverse transcriptase polymerase chain reaction (RT-PCR) and Western Blotting. The miR-143 lentiviral vector was successfully stably transfected in HMC-1 cells for target gene expression. Compared to the control, the target gene IL-13Rα1 was less expressed in HMC-1 transfected with miR-143 as determined by RT-PCR and Western Blotting (p < 0.05); this difference in expression was statistically significant and the inhibition efficiency was 71%. It indicates that miR-143 directly targets IL-13Rα1 and suppresses IL-13Rα1 expression in HMC-1 cells. Therefore, miR-143 may be associated with allergic reaction in human mast cells.

【 授权许可】

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© 2013 by the authors; licensee MDPI, Basel, Switzerland