Micromachines | |
Lysis of a Single Cyanobacterium for Whole Genome Amplification | |
Eric W. Hall1  Samuel Kim1  Visham Appadoo1  | |
[1] Department of Chemistry, Stanford University, Stanford, CA 94305, USA; E-Mail: | |
关键词: cyanobacteria; lysis; whole genome amplification; single cell; microfluidics; | |
DOI : 10.3390/mi4030321 | |
来源: mdpi | |
【 摘 要 】
Bacterial species from natural environments, exhibiting a great degree of genetic diversity that has yet to be characterized, pose a specific challenge to whole genome amplification (WGA) from single cells. A major challenge is establishing an effective, compatible, and controlled lysis protocol. We present a novel lysis protocol that can be used to extract genomic information from a single cyanobacterium of
【 授权许可】
CC BY
© 2013 by the authors; licensee MDPI, Basel, Switzerland.
【 预 览 】
Files | Size | Format | View |
---|---|---|---|
RO202003190033835ZK.pdf | 733KB | download |