Enhancement of Cunninghamella elegans UCP/WFCC 0542 Biomass and Chitosan with Amino Acid Supply

Molecules

Ednaldo Ramos dos Santos¹  Marta Cristina Freitas da Silva⁴  Patrໜia Mendes de Souza²  Antonio Cardoso da Silva³  Sergio Carvalho de Paiva⁵  Clarissa D. C. Albuquerque²  Aline E. Nascimento²  Kaoru Okada² 

[1] Biological Sciences Program, Federal University of Pernambuco, Recife 50670-901, PE, Brazil; E-Mail:;Nucleus of Research in Environmental Sciences, General Coordination of Research, Catholic University of Pernambuco, Recife 50050-590, PE, Brazil; E-Mails:;Department of Education, State of Pernambuco, 50.810-000 Recife, PE, Brazil; E-Mail:;Center of Biodiversity Studies, Federal University of Roraima, 69309-270 Boa Vista, RR, Brazil; E-Mails:;Center of Sciences and Technology, University of Pernambuco, Recife 50050-900, PE, Brazil; E-Mail:

关键词: Cunninghamella elegans;    chitosan;    sucrose;    asparagine;    corn steep liquor;   

DOI  :  10.3390/molecules180910095

来源: mdpi

【 摘 要 】

Studies were carried out with Cunninghamella elegans UCP/WFCC 0542 to evaluate the effects of an abundant supply of amino acids, asparagine and corn steep liquor associated with sucrose on the production of biomass and chitosan by submerged fermentation. The concentrations of the components of the culture medium which were determined by a 2³ full factorial design evaluated the interactions and effects of the independent variables of the sucrose, asparagine and corn steep liquor in relation to carbon and nitrogen sources, on the production of chitosan regarding biomass. The best results were observed at the central point [asparagine 0.025%, sucrose 0.15% and 0.45% of corn steep liquor, ratio C:N=2:6], and produced maximum yields of 16.95 g/L biomass and 2.14 g/L chitosan, after 96 h of submerged fermentation. However, the lowest level of sucrose, asparagine and corn steep liquor produced a low amount of biomass (10.83 g/L) and chitosan (0.60g/L). The infrared spectrum absorption of the chitosan produced by C. elegans showed bands regarding OH-axial stretching between 3406 and 3432 cm⁻¹, superimposed on the NH stretching band with axial deformation of the amide C=O group at about 1639 cm⁻¹, NH angular deformation at approximately 1560 cm⁻¹; axial deformation of amide-CN at around 1421 cm⁻¹, symmetrical angular deformation in CH₃ at 1379 cm⁻¹, -CN axial deformation of amino groups from 1125 to 1250 cm⁻¹ and polysaccharide structure bands in the range of between 890–1150 cm⁻¹. The crystallinity index of chitosan was 60.92%, and its degree of deacetylation was 75.25%. A low percentage of a supply of sucrose and asparagine with corn steep liquor offered higher yields of biomass and chitosan production at low cost.

【 授权许可】

CC BY   
© 2013 by the authors; licensee MDPI, Basel, Switzerland.