期刊论文详细信息
Molecules
Pancreatic Lipase Inhibitory Gallotannins from Galla Rhois with Inhibitory Effects on Adipocyte Differentiation in 3T3-L1 Cells
O Jun Kwon1  Jong-Sup Bae2  Ha Yeong Lee2  Ju-Young Hwang3  Eun-Woo Lee4  Hideyuki Ito5 
[1] Daegyeong Institute for Regional Program Evaluation, Gyeongsan 712-210, Korea; E-Mail:;College of Pharmacy, Research Institute of Pharmaceutical Sciences, Kyungpook National University, Daegu 702-701, Korea; E-Mails:;Department of Food Biotechnology, Andong National University, Andong 760-749, Korea; E-Mail:;Department of Life Science and Biotechnology, Dongeui University, Busan 614-714, Korea; E-Mail:;Faculty of Health and Welfare Sciences, Okayama Prefectural University, Okayama 719-1197, Japan; E-Mails:
关键词: Galla Rhois;    gallotannins;    pancreatic lipase;    protoaphin-fb;    3T3-L1;    2-O-digalloyl-1;    3;    4;    6-tetra-O-galloyl-β-d-glucose;   
DOI  :  10.3390/molecules180910629
来源: mdpi
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【 摘 要 】

Activity-guided isolation of a methanolic extract of Galla Rhois using pancreatic lipase and 3T3-L1 adipocytes led to the isolation of seven phenolic compounds: protoaphin-fb (1), 2-O-digalloyl-1,3,4,6-tetra-O-galloyl-β-d-glucose (2), 1,2,3,4,6-penta-O-galloyl-β-d-glucose (3), 1,2,4,6-tetra-O-galloyl-β-d-glucose (4), 3-hydroxy-5-methoxy-phenol 1-O-β-d-glucoside (5), methylgallate (6), and gallic acid (7). Their structures were established on the basis of NMR and MS spectroscopic data interpretation. All isolates were evaluated for their inhibitory effects on pancreatic lipase, and compounds 1-5 exhibited potent inhibitory effects on this enzyme, with IC50 values ranging from 30.6 ± 2.4 to 3.5 ± 0.5 μM. In addition, the highly galloylated compound 2 was also found to induce potent inhibition of adipocyte differentiation in 3T3-L1 cells.

【 授权许可】

CC BY   
© 2013 by the authors; licensee MDPI, Basel, Switzerland.

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