Effect of Black Grape Juice against Heart Damage from Acute Gamma TBI in Rats

Molecules

Robson Borba de Freitas³  Aline Augusti Boligon³  Bruno Tomazele Rovani³  Mariana Piana³  Thiele Faccim de Brum³  Roberta da Silva Jesus³  Fagner Chagas Rother⁴  Nelson Mendes Alves¹  João Batista Teixeira da Rocha²  Margareth Linde Athayde⁵  Juan Pablo Barrio⁶  Edson Ramos de Andrade⁴ 

[1] Postgraduate Program, Institute of Nuclear and Energy Research of University of São Paulo (IPEN), São Paulo, SP, 05508-000, Brazil;Department of Chemistry, Center of Natural and Exact Sciences, Federal University of Santa Maria (UFSM), Santa Maria, RS, 97105-900, Brazil;Postgraduate Program in Pharmaceutical Sciences, Center of Health Sciences, Federal University of Santa Maria (UFSM), Santa Maria, RS, 97105-900, Brazil;Nuclear Engineering Program, Military Institute of Engineering (IME), Rio de Janeiro, RJ, 22290-270, Brazil;Department of Industrial Pharmacy, Center of Health Sciences, Federal University of Santa Maria (UFSM), Santa Maria, RS, 97105-900, Brazil;Department of Biomedical Sciences and Institute of Biomedicine (IBIOMED), University of León, León 24071, Spain

关键词: acute radiation syndrome;    black grape juice;    heart;    oxidative damage;    lactate dehydrogenase;    phenolics;    flavonoids;    HPLC-DAD;   

DOI  :  10.3390/molecules181012154

来源: mdpi

【 摘 要 】

The aim of this study was to evaluate the potential positive effect of black grape juice (BGJ) on lipid peroxidation considering Total Body Irradiation (TBI) in Wistar rats. As a potential feasible means of evaluation in situ, blood serum lactate dehydrogenase (LDH) levels were evaluated as a marker for heart damage from acute radiation syndrome (ARS). Twenty rats were divided into four groups, two of them being irradiated by gamma-rays from a Co-60 source. Animals were treated by gavage with 2 mL per day of BGJ or placebo for one week before and 4 days after 6 Gy whole body gamma-irradiation, when they were euthanasiated. LDH on serum and lipid peroxidation on heart tissue were evaluated. High concentration of metabolites from lipid peroxidation in heart, and high LDH level on serum were found only in gamma-irradiated group given placebo, mainly at the first 24 h after radiation. Phytochemical analysis of BGJ was performed by determining total phenolics, flavonoids, and tannins followed by a high-performance liquid chromatography (HPLC/DAD) analysis, which showed resveratrol as the major constituent. Results suggest that BGJ is a good protective candidate compound against heart damage from ARS and its effects suggest its use as a radiomodifier.

【 授权许可】

CC BY   
© 2013 by the authors; licensee MDPI, Basel, Switzerland.