Direct Light-up of cAMP Derivatives in Living Cells by Click Reactions

Molecules

Kenichiro Ito²  Hongshan Liu¹  Makoto Komiyama²  Tetsuya Hayashi³ 

[1] Division of Chemistry, Department of Medical Sciences Faculty of Medicine, University of Miyazaki 5200 Kihara, Kiyotake, Miyazaki 889-1692, Japan;Research Center for Advanced Science and Technology, The University of Tokyo 4-6-1 Komaba, Meguro-ku, Tokyo 153-8904, Japan;Division of Microbial Genomics, Department of Genomics and Bioenvironmental Science, Frontier Science Research Center, and Division of Microbiology, Department of Infectious Diseases, Faculty of Medicine, University of Miyazaki, 5200 Kiyotake, Miyazaki 889-1692, Japan

关键词: click chemistry;    cAMP;    fluorescence;    cAMP localization in living cells;   

DOI  :  10.3390/molecules181012909

来源: mdpi

【 摘 要 】

8-Azidoadenosine 3′,5′-cyclic monophosphate (8-azido cAMP) was directly detected in living cells, by applying Cu-free azide-alkyne cycloaddition to probe cAMP derivatives by fluorescence light-up. Fluorescence emission was generated by two non-fluorescent molecules, 8-azido cAMP as a model target and difluorinated cyclooctyne (DIFO) reagent as a probe. The azide-alkyne cycloaddition reaction between 8-azido cAMP and DIFO induces fluorescence in 8-azido cAMP. The fluorescence emission serves as a way to probe 8-azido cAMP in cells.

【 授权许可】

CC BY   
© 2013 by the authors; licensee MDPI, Basel, Switzerland.