Quorum Sensing Activity of Enterobacter asburiae Isolated from Lettuce Leaves

Sensors

Yin Yin Lau¹  Joanita Sulaiman¹  Jian Woon Chen¹  Wai-Fong Yin¹ 

[1] Division of Genetics and Molecular Biology, Institute of Biological Sciences, Faculty of Science, University of Malaya, Kuala Lumpur 50603, Malaysia;

关键词: Enterobacter asburiae;    MALDI-TOF;    mass spectrometry;    N-acyl homoserine lactone;    N—hexanoyl homoserine lactone;    N-butanoyl homoserine lactone;    quorum sensing;    lettuce;    food spoilage;    food microbiology;    food safety;   

DOI  :  10.3390/s131014189

来源: mdpi

【 摘 要 】

Bacterial communication or quorum sensing (QS) is achieved via sensing of QS signaling molecules consisting of oligopeptides in Gram-positive bacteria and N-acyl homoserine lactones (AHL) in most Gram-negative bacteria. In this study, Enterobacteriaceae isolates from Batavia lettuce were screened for AHL production. Enterobacter asburiae, identified by matrix-assisted laser desorption ionization time of flight mass spectrometry (MALDI-TOF-MS) was found to produce short chain AHLs. High resolution triple quadrupole liquid chromatography mass spectrometry (LC/MS) analysis of the E. asburiae spent supernatant confirmed the production of N-butanoyl homoserine lactone (C4-HSL) and N–hexanoyl homoserine lactone (C6-HSL). To the best of our knowledge, this is the first report of AHL production by E. asburiae.

【 授权许可】

CC BY   
© 2013 by the authors; licensee MDPI, Basel, Switzerland.