Detection of Quorum Sensing Activity in the Multidrug-Resistant Clinical Isolate Pseudomonas aeruginosa Strain GB11

Sensors

Huey Jia Cheng¹  Kok-Gan Chan¹  Wai-Fong Yin¹  Wen-Si Tan¹  Robson Ee¹  Yuet Meng Cheong² 

[1] Division of Genetics and Molecular Biology, Institute of Biological Sciences, Faculty of Science, University of Malaya, Kuala Lumpur 50603, Malaysia;Jeffrey Cheah School of Medicine & Health Sciences, Monash University Malaysia, Jalan Lagoon Selatan, 46150 Bandar Sunway, Selangor Darul Ehsan, Malaysia;

关键词: MALDI-TOF;    mass spectrometry;    N-butyryl homoserine lactone (C4-HSL);    N-hexanoyl homoserine lactone (C6-HSL);    N-octanoyl homoserine lactone (C8-HSL);    N-dodecanoyl homoserine lactone (C12-HSL);    N-3-oxo-dodecanoylhomoserine lactone(3-oxo-C12-HSL);    clinical pathogen;    cell-to-cell communication;   

DOI  :  10.3390/s140712511

来源: DOAJ

【 摘 要 】

A multidrug-resistant clinical bacteria strain GB11 was isolated from a wound swab on the leg of a patient. Identity of stain GB11 as Pseudomonas aeruginosa was validated by using matrix-assisted laser desorption/ionization-time-of-flight mass spectrometry (MALDI-TOF MS). Detection of the production of signaling molecules,N-acylhomoserine lactones (AHLs), was conducted using three different bacterial biosensors. A total of four different AHLs were found to be produced by strain GB11, namely N-butyryl homoserine lactone (C4-HSL), N-hexanoylhomoserine lactone (C6-HSL), N-octanoyl homoserine lactone (C8-HSL) and N-3-oxo-dodecanoylhomoserine lactone(3-oxo-C12-HSL) using high resolution liquid chromatography tandem mass spectrometry (LC-MS/MS). Of these detected AHLs, 3-oxo-C12-HSL was found to be the most abundant AHL produced by P. aeruginosa GB11.

【 授权许可】

Unknown