Anti HSV-1 Activity of Halistanol Sulfate and Halistanol Sulfate C Isolated from Brazilian Marine Sponge Petromica citrina (Demospongiae)

Marine Drugs

Tatiana da Rosa Guimar฾s¹  Carlos Guillermo Quiroz⁴  Caroline Rigotto¹  Simone Quintana de Oliveira¹  Maria Tereza Rojo de Almeida¹  Éverson Miguel Bianco¹  Maria Izabel Goulart Moritz¹  João Luís Carraro³  Jorge Alejandro Palermo²  Gabriela Cabrera²  Eloir Paulo Schenkel¹  Flávio Henrique Reginatto¹ 

[1] Laboratory of Natural Products, Department of Pharmaceutical Sciences, Universidade Federal de Santa Catarina, Florianópolis 88040-900, SC, Brazil; E-Mails:;UMYMFOR—Department of Organic Chemistry, FCEN—University of Buenos Aires, Buenos Aires C1428EGA, Argentina; E-Mails:;Laboratory of Porifera, National Museum, Universidade Federal do Rio de Janeiro, Rio de Janeiro 20940-040, RJ, Brazil; E-Mail:;Laboratory of Applied Virology, Department of Microbiology, Immunology and Parasitology, Universidade Federal de Santa Catarina, Florianópolis 88040-900, SC, Brazil; E-Mails:

关键词: antiviral activity;    HSV-1;    marine sponge;    Petromica citrina;    sulfate sterols;   

DOI  :  10.3390/md11114176

来源: mdpi

【 摘 要 】

The n-butanol fraction (BF) obtained from the crude extract of the marine sponge Petromica citrina, the halistanol-enriched fraction (TSH fraction), and the isolated compounds halistanol sulfate (1) and halistanol sulfate C (2), were evaluated for their inhibitory effects on the replication of the Herpes Simplex Virus type 1 (HSV-1, KOS strain) by the viral plaque number reduction assay. The TSH fraction was the most effective against HSV-1 replication (SI = 15.33), whereas compounds 1 (SI = 2.46) and 2 (SI = 1.95) were less active. The most active fraction and these compounds were also assayed to determine the viral multiplication step(s) upon which they act as well as their potential synergistic effects. The anti-HSV-1 activity detected was mediated by the inhibition of virus attachment and by the penetration into Vero cells, the virucidal effect on virus particles, and by the impairment in levels of ICP27 and gD proteins of HSV-1. In summary, these results suggest that the anti-HSV-1 activity of TSH fraction detected is possibly related to the synergic effects of compounds 1 and 2.

【 授权许可】

CC BY   
© 2013 by the authors; licensee MDPI, Basel, Switzerland.