Antiviral Sulfoquinovosyldiacylglycerols (SQDGs) from the Brazilian Brown Seaweed Sargassum vulgare

Marine Drugs

Erwan Plouguerné¹  Lauro M. de Souza²  Guilherme L. Sassaki²  Jéssica Figueiredo Cavalcanti³  Maria Teresa Villela Romanos³  Bernardo A. P. da Gama¹  Renato Crespo Pereira¹ 

[1] Department of Marine Biology, Biology Institute, Fluminense Federal University, Niterói 24210-130, RJ, Brazil; E-Mails:;Department of Biochemistry and Molecular Biology, Paraná Federal University, Curitiba 81531-990, PR, Brazil; E-Mails:;Department of Virology, Microbiology Institute, Federal University of Rio de Janeiro, Rio de Janeiro 21941-902, RJ, Brazil; E-Mails:

关键词: Sargassum vulgare;    glycolipids;    sulfoquinovosyldiacylglycerol;    anti-HSV activity;    palmitic acid;   

DOI  :  10.3390/md11114628

来源: mdpi

【 摘 要 】

Total lipids from the Brazilian brown seaweed Sargassum vulgare were extracted with chloroform/methanol 2:1 and 1:2 (v/v) at room temperature. After performing Folch partition of the crude lipid extract, the lipids recovered from the Folch lower layer were fractionated on a silica gel column eluted with chloroform, acetone and methanol. The fraction eluted with methanol, presented a strong orcinol-positive band characteristic of the presence of sulfatides when examined by TLC. This fraction was then purified by two successive silica gel column chromatography giving rise to fractions F4I86 and F4II90 that exhibited strong activity against herpes simplex virus type 1 and 2. The chemical structures present in both fractions were elucidated by ESI-MS and ¹H/¹³C NMR analysis HSQC fingerprints based on their tandem–MS behavior as sulfoquinovosildiacylglycerols (SQDGs). The main SQDG present in both fractions and responsible for the anti-herpes activity observed was identified as 1,2-di-O-palmitoyl-3-O-(6-sulfo-α-d-quinovopyranosyl)-glycerol.

【 授权许可】

CC BY   
© 2013 by the authors; licensee MDPI, Basel, Switzerland.