Semiconductor Nanomaterials-Based Fluorescence Spectroscopic and Matrix-Assisted Laser Desorption/Ionization (MALDI) Mass Spectrometric Approaches to Proteome Analysis

Materials

Suresh Kumar Kailasa²  Kuang-Hung Cheng¹ 

[1]Institute of Biomedical Sciences, National Sun Yat-Sen University, Kaohsiung 804, Taiwan

[2] E-Mail:

[3]Department of Chemistry, S. V. National Institute of Technology, Surat 395007, India

[4] E-Mail:

关键词: quantum dots;    fluorescent and affinity probes;    biomolecules;    fluorescence spectroscopy;    MALDI-MS;   

DOI  :  10.3390/ma6125763

来源: mdpi

【 摘 要 】

Semiconductor quantum dots (QDs) or nanoparticles (NPs) exhibit very unusual physico-chemcial and optical properties. This review article introduces the applications of semiconductor nanomaterials (NMs) in fluorescence spectroscopy and matrix-assisted laser desorption/ionization mass spectrometry (MALDI-MS) for biomolecule analysis. Due to their unique physico-chemical and optical properties, semiconductors NMs have created many new platforms for investigating biomolecular structures and information in modern biology. These semiconductor NMs served as effective fluorescent probes for sensing proteins and cells and acted as affinity or concentrating probes for enriching peptides, proteins and bacteria proteins prior to MALDI-MS analysis.

【 授权许可】

CC BY   
© 2013 by the authors; licensee MDPI, Basel, Switzerland.