Estimating Cyanobacteria Community Dynamics and its Relationship with Environmental Factors

International Journal of Environmental Research and Public Health

Wenhuai Luo¹  Huirong Chen¹  Anping Lei¹  Jun Lu¹ 

[1] Shenzhen Key Laboratory of Marine Bioresource and Eco-environmental Science, Shenzhen Engineering Laboratory of Marine Algal Biotechnology, College of Life Science, Shenzhen University, Shenzhen 518060, China; E-Mails:

关键词: eutrophication;    cyanobacteria community composition;    PCR-DGGE;    freshwater lakes;   

DOI  :  10.3390/ijerph110101141

来源: mdpi

【 摘 要 】

The cyanobacteria community dynamics in two eutrophic freshwater bodies (Tiegang Reservoir and Shiyan Reservoir) was studied with both a traditional microscopic counting method and a PCR-DGGE genotyping method. Results showed that cyanobacterium Phormidium tenue was the predominant species; twenty-six cyanobacteria species were identified in water samples collected from the two reservoirs, among which fourteen were identified with the morphological method and sixteen with the PCR-DGGE method. The cyanobacteria community composition analysis showed a seasonal fluctuation from July to December. The cyanobacteria population peaked in August in both reservoirs, with cell abundances of 3.78 × 10⁸ cells L^-1 and 1.92 × 10⁸ cells L^-1 in the Tiegang and Shiyan reservoirs, respectively. Canonical Correspondence Analysis (CCA) was applied to further investigate the correlation between cyanobacteria community dynamics and environmental factors. The result indicated that the cyanobacteria community dynamics was mostly correlated with pH, temperature and total nitrogen. This study demonstrated that data obtained from PCR-DGGE combined with a traditional morphological method could reflect cyanobacteria community dynamics and its correlation with environmental factors in eutrophic freshwater bodies.

【 授权许可】

CC BY   
© 2014 by the authors; licensee MDPI, Basel, Switzerland.