Effects of Bread Making and Wheat Germ Addition on the Natural Deoxynivalenol Content in Bread

Toxins

Isabel Giménez²  Jesús Blesa¹  Marta Herrera² 

[1] Faculty of Pharmacy, University of Valencia, Burjassot 46100, Valencia, Spain; E-Mail:;Veterinary Faculty, University of Zaragoza, Zaragoza 50013, Spain; E-Mails:

关键词: deoxynivalenol;    bread making;    wheat germ;   

DOI  :  10.3390/toxins6010394

来源: mdpi

【 摘 要 】

Deoxynivalenol (DON, vomitoxin) is a type-B trichothecene mycotoxin produced by several field fungi such as Fusarium graminearum and Fusarium culmorum and known to have various toxic effects. This study investigated the effect of the bread making process on the stability of DON in common bread and wheat germ-enriched bread using naturally contaminated ingredients at the level of 560 µg/kg. The concentration of DON and its evolution during bread making were determined by immunoaffinity column cleanup followed by liquid chromatography with diode array detection (HPLC-DAD). During the bread making process, DON was reduced by 2.1% after fermentation and dropped by 7.1% after baking, reaching a maximum reduction of 19.8% in the crust as compared with a decrease of 5.6% in the crumb. The addition of 15% wheat germ to the dough did not affect DON stability during bread making, showing an apparent increase of 3.5% after fermentation and a reduction by 10.2% after baking.

【 授权许可】

CC BY   
© 2014 by the authors; licensee MDPI, Basel, Switzerland.