Protein A Detection Based on Quantum Dots-Antibody Bioprobe Using Fluorescence Coupled Capillary Electrophoresis

International Journal of Molecular Sciences

Lin Qiu¹  Yanhua Bi³  Cheli Wang²  Jingyan Li²  Peilin Guo²  Jinchen Li²  Weijiang He¹  Jianhao Wang² 

[1] State Key Laboratory of Coordination Chemistry, Coordination Chemistry Institute, School of Chemistry and Chemical Engineering, Nanjing University, Nanjing 210093, China; E-Mails:;School of Pharmaceutical Engineering and Life Science, Changzhou University, Changzhou 213164, China; E-Mails:;Shandong Academy of Medical Sciences, Jinan 250062, China; E-Mail:

关键词: QDs;    FRET;    bioprobe;    capillary electrophoresis;    protein A;   

DOI  :  10.3390/ijms15021804

来源: mdpi

【 摘 要 】

In this report, fluorescence detection coupled capillary electrophoresis (CE-FL) was used to detect Protein A. Antibody was first labeled with Cy5 and then mixed with quantum dots (QDs) to form QDs-antibody bioprobe. Further, we observed fluorescence resonance energy transfer (FRET) from QDs donor to Cy5 acceptor. The bioprobe was formed and brought QDs and Cy5 close enough to allow FRET to occur. After adding protein A, the FRET system was broken and caused the FRET signal to decrease. Thus, a new method for the determination of protein A was proposed based on the FRET signal changes. This study provides a new trail of thought for the detection of protein.

【 授权许可】

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