Expression of a Deschampsia antarctica Desv. Polypeptide with Lipase Activity in a Pichia pastoris Vector

International Journal of Molecular Sciences

Claudia Rabert²  Ana Gutiérrez-Moraga²  Alejandro Navarrete-Gallegos¹  Darío Navarrete-Campos¹  León A. Bravo² 

[1] Laboratorio de Fisiología Vegetal, Departamento de Botánica, Universidad de Concepción, Casilla 160-C, Correo 3, Concepción 3801061, Chile; E-Mails:;Laboratorio de Fisiología y Biología Molecular Vegetal, Instituto de Agroindustria, Facultad de Ciencias Agropecuarias y Forestales, Universidad de La Frontera, Casilla 54D, Temuco 4811230, Chile; E-Mails:

关键词: Deschampsia antarctica;    lipase;    Pichia pastoris;    detergent tolerance;   

DOI  :  10.3390/ijms15022359

来源: mdpi

【 摘 要 】

The current study isolated and characterized the Lip3F9 polypeptide sequence of Deschampsia antarctica Desv. (GeneBank Accession Number JX846628), which was found to be comprised of 291 base pairs and was, moreover, expressed in Pichia pastoris X-33 cells. The enzyme was secreted after 24 h of P. pastoris culture incubation and through induction with methanol. The expressed protein showed maximum lipase activity (35 U/L) with an optimal temperature of 37 °C. The lipase-expressed enzyme lost 50% of its specific activity at 25 °C, a behavior characteristic of a psychrotolerant enzyme. Recombinant enzyme activity was measured in the presence of ionic and non-ionic detergents, and a decrease in enzyme activity was detected for all concentrations of ionic and non-ionic detergents assessed.

【 授权许可】

CC BY   
© 2014 by the authors; licensee MDPI, Basel, Switzerland