Cloning and Expression of Aspergillus tamarii FS132 Lipase Gene in Pichia pastoris

International Journal of Molecular Sciences

Bihong Shi²  Liqing Zeng²  Haolei Song²  Qiaoqin Shi¹ 

[1] Engineering Research Center of Industrial Microbiology, Ministry of Education, Fujian Normal University, Fuzhou 350108, China; E-Mails:;College of Life Sciences, Fujian Normal University, The University Town, Min-hou, Fuzhou 350108, China; E-Mails:

关键词: Aspergillus tamarii;    lipase;    gene cloning;    expression;    Pichia pastoris;   

DOI  :  10.3390/ijms11062373

来源: mdpi

【 摘 要 】

A lipase gene (atl) was cloned from Aspergillus tamarii FS132 for the first time. The gene was found to have an open reading frame of 1024 base pairs (bp), and the coding region of the gene contained two introns (51 bp and 52 bp). Multi-alignment analysis of the deduced amino acid sequence indicated high homology between the enzyme and mono-and diacylglycerol lipases from fungi Aspergillus. The recombinant lipase was expressed in Pichia pastoris GS115 cells. The recombinant lipase was found to have a molecular mass of 36.7 kDa, and it exhibited lipase activity of 20 U/mL in culture supernatant when tributyrin was used as the substrate.

【 授权许可】

CC BY   
© 2010 by the authors; licensee Molecular Diversity Preservation International, Basel, Switzerland.