Bacterial Cellular Engineering by Genome Editing and Gene Silencing

International Journal of Molecular Sciences

Nobutaka Nakashima¹ 

[1] Bioproduction Research Institute, National Institute of Advanced Industrial Sciences and Technology (AIST), 2-17-2-1 Tsukisamu-Higashi, Toyohira-ku, Sapporo 062-8517, Japan; E-Mail:

关键词: gene knockout;    allelic exchange;    gene knock-in;    genome editing;    RNA-guided nucleases;    mobile group II intron;    antisense RNA;    gene knockdown;    gene silencing;   

DOI  :  10.3390/ijms15022773

来源: mdpi

【 摘 要 】

Genome editing is an important technology for bacterial cellular engineering, which is commonly conducted by homologous recombination-based procedures, including gene knockout (disruption), knock-in (insertion), and allelic exchange. In addition, some new recombination-independent approaches have emerged that utilize catalytic RNAs, artificial nucleases, nucleic acid analogs, and peptide nucleic acids. Apart from these methods, which directly modify the genomic structure, an alternative approach is to conditionally modify the gene expression profile at the posttranscriptional level without altering the genomes. This is performed by expressing antisense RNAs to knock down (silence) target mRNAs in vivo. This review describes the features and recent advances on methods used in genomic engineering and silencing technologies that are advantageously used for bacterial cellular engineering.

【 授权许可】

CC BY   
© 2014 by the authors; licensee MDPI, Basel, Switzerland