Molecules | |
Pharmacophore Generation from a Drug-like Core Molecule Surrounded by a Library Peptide via the 10BASEd-T on Bacteriophage T7 | |
Yuuki Tokunaga1  Yuuki Azetsu1  Keisuke Fukunaga1  Takaaki Hatanaka2  Yuji Ito2  | |
[1] Department of Engineering Science, Bioscience and Technology Program, The Graduate School of Informatics and Engineering, The University of Electro-Communications (UEC), 1-5-1 Chofugaoka, Chofu, Tokyo 182-8585, Japan; E-Mails:;Department of Chemistry and Bioscience, Graduate School of Science and Engineering, Kagoshima University, 1-21-35 Korimoto, Kagoshima, Kagoshima 890-0065, Japan; E-Mails: | |
关键词: 10BASEd-T; bacteriophage T7; chemical modification; drug-like molecule; peptide library; phage display; pharmacophore; salicylic acid; site-specific conjugation; thioether; | |
DOI : 10.3390/molecules19022481 | |
来源: mdpi | |
【 摘 要 】
We have achieved site-specific conjugation of several haloacetamide derivatives into designated cysteines on bacteriophage T7-displayed peptides, which are fused to T7 capsid protein gp10. This easiest gp10 based-thioetherification (10BASEd-T) undergoes almost quantitatively like a click reaction without side reaction or loss of phage infectivity. The post-translational modification yield, as well as the site-specificity, is quantitatively analyzed by a fluorescent densitometric analysis after gel electrophoresis. The detailed structure of the modified peptide on phage is identified with tandem mass spectrometry. Construction of such a peptide-fused phage library possessing non-natural core structures will be useful for future drug discovery. For this aim, we propose a novel concept of pharmacophore generation from a drug-like molecule (
【 授权许可】
CC BY
© 2014 by the authors; licensee MDPI, Basel, Switzerland.
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