Prevalence and Antimicrobial Resistance of Enterococcus Species: A Hospital-Based Study in China

International Journal of Environmental Research and Public Health

Wei Jia¹  Gang Li² 

[1] Medical Experimental Center, General Hospital of Ningxia Medical University, 804 Shengli Street, Yinchuan City 750004, Ningxia Hui Autonomous Region, China;

关键词: Enterococcus spp.;    antimicrobial resistance;    active efflux mechanism;    reserpine;    fluoroquinolones;   

DOI  :  10.3390/ijerph110303424

来源: mdpi

【 摘 要 】

Objective: to investigate the prevalence and antimicrobial resistance of Enterococcus species isolated from a university hospital, and explore the mechanisms underlying the antimicrobial resistance, so as to provide clinical evidence for the inappropriate clinical use of antimicrobial agents and the control and prevention of enterococcal infections. Methods: a total of 1,157 enterococcal strains isolated from various clinical specimens from January 2010 to December 2012 in the General Hospital of Ningxia Medical University were identified to species level with a VITEK-2 COMPACT fully automated microbiological system, and the antimicrobial susceptibility of Enterococcus species was determined using the Kirby-Bauer disc diffusion method. The multiple-drug resistant enterococcal isolates were screened from the clinical isolates of Enterococcus species from the burns department. The minimal inhibitory concentration (MIC) of Enterococcus species to the three fluoroquinolones, including ciprofloxacin, gatifloxacin and levofloxacin was determined with the agar dilution method, and the changes in the MIC of Enterococcus species to the three fluoroquinolones following reserpine treatment were evaluated. The β-lactam, aminoglycoside, tetracycline, macrolide, glycopeptide resistance genes and the efflux pump emeA genes were detected in the enterococcal isolates using a polymerase chain reaction (PCR) assay. Results: the 1,157 clinical isolates of Enterococcus species included 679 E. faecium isolates (58.7%), 382 E. faecalis isolates (33%), 26 E. casseliflavus isolates (2.2%), 24 E. avium isolates (2.1%), and 46 isolates of other Enterococcus species (4%).

【 授权许可】

CC BY   
© 2014 by the authors; licensee MDPI, Basel, Switzerland.