Asynchronous Magnetic Bead Rotation (AMBR) Microviscometer for Label-Free DNA Analysis

Biosensors

Yunzi Li¹  David T. Burke³  Raoul Kopelman² 

[1] Department of Chemical Engineering, University of Michigan, Ann Arbor, MI 48109, USA; E-Mail:;Department of Chemistry, University of Michigan, Ann Arbor, MI 48109, USA;Department of Human Genetics, University of Michigan, Ann Arbor, MI 48109, USA; E-Mail:

关键词: label-free;    DNA;    quantitative PCR;    restriction digestion;    genetic analysis;    viscosity;    viscometer;    magnetic;    paramagnetic;    microdevice;   

DOI  :  10.3390/bios4010076

来源: mdpi

【 摘 要 】

We have developed a label-free viscosity-based DNA detection system, using paramagnetic beads as an asynchronous magnetic bead rotation (AMBR) microviscometer. We have demonstrated experimentally that the bead rotation period is linearly proportional to the viscosity of a DNA solution surrounding the paramagnetic bead, as expected theoretically. Simple optical measurement of asynchronous microbead motion determines solution viscosity precisely in microscale volumes, thus allowing an estimate of DNA concentration or average fragment length. The response of the AMBR microviscometer yields reproducible measurement of DNA solutions, enzymatic digestion reactions, and PCR systems at template concentrations across a 5000-fold range. The results demonstrate the feasibility of viscosity-based DNA detection using AMBR in microscale aqueous volumes.

【 授权许可】

CC BY   
© 2014 by the authors; licensee MDPI, Basel, Switzerland.