Fabrication of Calix[4]arene Derivative Monolayers to Control Orientation of Antibody Immobilization

International Journal of Molecular Sciences

Hongxia Chen¹  Feng Liu¹  Fangjie Qi¹  Kwangnak Koh² 

[1] Laboratory of Biosensing Technology, School of Life Sciences, Shanghai University, Shanghai 200444, China; E-Mails:;College of Nanoscience and Nanotechnology, Pusan National University, Pusan 609-735, Korea; E-Mail:

关键词: calixarene;    surface plasmon resonance (SPR);    Immunoglobulin G (IgG);    orientation;    dipole moment;   

DOI  :  10.3390/ijms15045496

来源: mdpi

【 摘 要 】

Three calix[4]arene (Cal-4) derivatives which separately contain ethylester (1), carboxylic acid (2), and crownether (3) at the lower rim with a common reactive thiol at the upper rim were synthesized and constructed to self-assembled monolayers (SAMs) on Au films. After spectroscopic characterization of the monolayers, surface coverage and orientation of antibody immobilized on the Cal-4 derivative SAMs were studied by surface plasmon resonance (SPR) technique. Experimental results revealed that the antibody could be immobilized on the Cal-4 derivatives spontaneously. The orientation of absorbed antibody on the Cal-4 derivative SAMs is related to the SAM’s dipole moment. The possible orientations of the antibody immobilized on the Cal-4 derivative 1 SAM are lying-on or side-on, while on the Cal-4 derivative 2 and Cal-4 derivative 3 head-on and end-on respectively. These experimental results demonstrate the surface dipole moment of Cal-4 derivative appears to be an important factor to antibody orientation. Cal-4 derivatives are useful in developing site direct protein chips.

【 授权许可】

CC BY   
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