Isolation and Characterization of a Novel Rebaudioside M Isomer from a Bioconversion Reaction of Rebaudioside A and NMR Comparison Studies of Rebaudioside M Isolated from Stevia rebaudiana Bertoni and Stevia rebaudiana Morita

Biomolecules

Indra Prakash¹  Cynthia Bunders¹  Krishna P. Devkota²  Romila D. Charan²  Catherine Ramirez²  Christopher Priedemann² 

[1] The Coca-Cola Company, Atlanta, GA 30313, USA; E-Mail:;AMRI-Albany, Analytical Development, Albany, NY 12212, USA; E-Mails:

关键词: Stevia rebaudiana Bertoni;    Stevia rebaudiana Morita;    rebaudioside M;    rebaudioside D;    rebaudioside M2;    bioconversion;    steviol glycosides;    structure characterization;    nuclear magnetic resonance (NMR);   

DOI  :  10.3390/biom4020374

来源: mdpi

【 摘 要 】

A minor product, rebaudioside M2 (2), from the bioconversion reaction of rebaudioside A (4) to rebaudioside D (3), was isolated and the complete structure of the novel steviol glycoside was determined. Rebaudioside M2 (2) is considered an isomer of rebaudioside M (1) and contains a relatively rare 1→6 sugar linkage. It was isolated and characterized with NMR (¹H, ¹³C, COSY, HSQC-DEPT, HMBC, 1D-TOCSY, and NOESY) and mass spectral data. Additionally, we emphasize the importance of 1D and 2D NMR techniques when identifying complex steviol glycosides. Numerous NMR spectroscopy studies of rebaudioside M (1), rebaudioside D (3), and mixture of 1 and 3 led to the discovery that SG17 which was previously reported in literature, is a mixture of rebaudioside D (3), rebaudioside M (1), and possibly other related steviol glycosides.

【 授权许可】

CC BY   
© 2014 by the authors; licensee MDPI, Basel, Switzerland.