On the Interaction of Adherent Cells with Thermoresponsive Polymer Coatings

Polymers

Katja Uhlig²  Hans G. Boerner¹  Erik Wischerhoff³  Jean-François Lutz³  Magnus S. Jaeger²  André Laschewsky³ 

[1] Max Planck Institute for Colloids and Interfaces, Potsdam 14476, Germany; E-Mail:;Fraunhofer Institute for Biomedical Engineering (IBMT), Potsdam 14476, Germany; E-Mails:;Fraunhofer Institute for Applied Polymer Research (IAP), Potsdam 14476, Germany; E-Mails:

关键词: thermoresponsive polymer;    RGD;    cell detachment;    polyethylene glycol;   

DOI  :  10.3390/polym6041164

来源: mdpi

【 摘 要 】

Thermoresponsive polymer coatings allow the control of adhesion of cells on synthetic substrates. In particular, decreasing the temperature below the lower critical solution temperature (LCST) of the polymer triggers the non-invasive detachment of cells from their cultivation substrate. Widening the range of applications of these coatings in cellular biotechnology requires a better understanding of their interaction with cells. By monitoring the morphological changes of cells during their detachment at various temperatures, we provide evidence that cell detachment is an active process. Analyses of cell residues that are left behind by the cells on the substrate during their detachment, further support this notion. In the second part of this work, we show that the kinetics of adhesion and the efficiency of detachment of cells can be controlled through the coadsorption of molecules bearing the peptide motif RGD (arginine-glycine-aspartic acid) with the polymers.

【 授权许可】

CC BY   
© 2014 by the authors; licensee MDPI, Basel, Switzerland.