Further Aspects of Ochratoxin A-Cation Interactions: Complex Formation with Zinc Ions and a Novel Analytical Application of Ochratoxin A-Magnesium Interaction in the HPLC-FLD System

Toxins

Miklós Poór²  Mónika Kuzma¹  Gergely Matisz³  Yin Li³  Pál Perjési¹  Sándor Kunsági-Máté³ 

[1] Department of Pharmaceutical Chemistry, University of Pécs, Pécs H-7624, Hungary; E-Mails:;Institute of Laboratory Medicine, University of Pécs, Pécs H-7624, Hungary; E-Mail:;Department of General and Physical Chemistry, University of Pécs, Pécs H-7624, Hungary; E-Mails:

关键词: ochratoxin A;    zinc ion;    magnesium ion;    fluorescence;    HPLC-FLD;   

DOI  :  10.3390/toxins6041295

来源: mdpi

【 摘 要 】

Ochratoxin A (OTA) is a mycotoxin produced by different Aspergillus and Penicillium species. Since its mechanism of action is not fully understood yet, it is important to gain further insight into different interactions of OTA at the molecular level. OTA is found worldwide in many foods and drinks. Moreover, it can also be detected in human and animal tissues and body fluids, as well. Therefore, the development of highly sensitive quantitative methods for the determination of OTA is of utmost importance. OTA most likely forms complexes with divalent cations, both in cells and body fluids. In the present study, the OTA-zinc interaction was investigated and compared to OTA-magnesium complex formation using fluorescence spectroscopy and molecular modeling. Our results show that zinc(II) ion forms a two-fold higher stable complex with OTA than magnesium(II) ion. In addition, based on the enhanced fluorescence emission of OTA in its magnesium-bound form, a novel RP-HPLC-fluorescence detector (FLD) method was also established. Our results highlight that the application of magnesium chloride in alkaline eluents results in an approximately two-fold increase in sensitivity using the HPLC-FLD technique.

【 授权许可】

CC BY   
© 2014 by the authors; licensee MDPI, Basel, Switzerland.