Amplified Fluorescent Aptasensor for Ochratoxin A Assay Based on Graphene Oxide and RecJf Exonuclease

Toxins

卷:12

Dehui Xiong¹  Han Zhao¹  Ying Yan¹  Changbei Ma¹ 

[1] School of Life Sciences, Central South University, Changsha 410013, China;

关键词: fluorescence;    ochratoxin A;    RecJ_f exonuclease;    graphene oxide;    signal amplification;   

DOI  :  10.3390/toxins12110670

来源: DOAJ

【 摘 要 】

In this study, we developed an aptamer-based fluorescent sensing platform for the detection of ochratoxin A (OTA) based on RecJ_f exonuclease-assisted signal amplification and interaction between graphene oxide (GO) and the OTA aptamer (OTA-apt). After optimizing the experimental conditions, the present aptamer-based sensing system can exhibit excellent fluorescent response in the OTA assay, with a limit of detection of 0.07 ng/mL. In addition to signal amplification, this strategy is also highly specific for other interfering toxins. Furthermore, this aptasensor can be reliably used for assessing red wine samples spiked with different OTA concentrations (2.4, 6 and 20 ng/mL). The proposed assay plays an important role in the field of food safety and can be transformed for detecting other toxins by replacing the sequence that recognizes the aptamer.

【 授权许可】

Unknown