Two Ellagic Acids Isolated from Roots of Sanguisorba officinalis L. Promote Hematopoietic Progenitor Cell Proliferation and Megakaryocyte Differentiation

Molecules

Xiaoping Gao¹  Jianming Wu¹  Wenjun Zou¹ 

[1] College of Pharmacy, Chengdu University of Traditional Chinese Medicine, Chengdu, 86611-137, China; E-Mails:

关键词: Sanguisorba officinalis L;    3;    3';    O-β-d-xyloside"'>4-tri-O-methylellagic acid 4'-O-β-d-xyloside;    3;    3';    4-tri-O-methylellagic acid;    hematopoietic progenitor cells;    cell proliferation;    megakaryocyte differentiation;   

DOI  :  10.3390/molecules19045448

来源: mdpi

【 摘 要 】

Using a bioassay-directed chromatographic separation, two ellagic acids were obtained from the ethyl acetate extract of the roots of Sanguisorba officinalis L. On the basis of chemical and spectroscopic methods, the two ellagic acids were identified as 3,3',4-tri-O-methylellagic acid-4'-O-β-d-xyloside and 3,3',4-tri-O-methylellagic acid. Stimulation of cell proliferation was assayed in hematopoietic progenitor cells using the Cell Counting kit-8 method. The megakaryocyte differentiation was determined in human erythroleukemia (HEL) cells using Giemsa staining and flow cytometry analysis. The ellagic acids significantly stimulated the proliferation of Baf3/Mpl cells. Morphology analysis and megakaryocyte specific-marker CD41 staining confirmed that the ellagic acids induced megakaryocyte differentiation in HEL cells. This is the first time that 3,3',4-tri-O-methylellagic acid or 3,3',4-tri-O-methylellagic acid-4'-O-β-d-xyloside are reported to induce megakaryopoiesis, suggesting a class of small molecules which differ from others non-peptidyl, and appears to have potential for clinical development as a therapeutic agent for patients with blood platelet disorders.

【 授权许可】

CC BY   
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