Evaluation of Antioxidant and Antimicrobial Activities and Phenolic Profile for Hyssopus officinalis, Ocimum basilicum and Teucrium chamaedrys

Molecules

Laurian Vlase¹  Daniela Benedec⁴  Daniela Hanganu⁴  Grigore Damian³  Ioan Csillag³  Bogdan Sevastre²  Augustin C. Mot⁵  Radu Silaghi-Dumitrescu⁵ 

[1] Department of Pharmaceutical Technology and Biopharmaceutics, Iuliu Hatieganu University of Medicine and Pharmacy, 12 I. Creanga Street, Cluj-Napoca 400010, Romania; E-Mail:;Department of Physiopathology, University of Agricultural Sciences and Veterinary Medicine, 3-5 Mănăştur Street, Cluj-Napoca 400372, Romania; E-Mail:;Department of Physics, Babes-Bolyai University, 1 M. Kogalniceanu Street, Cluj-Napoca 400084, Romania; E-Mails:;Department of Pharmacognosy, Iuliu Hatieganu University of Medicine and Pharmacy, 12 I. Creanga Street, Cluj-Napoca 400010, Romania;Department of Chemistry and Chemical Engineering Babes-Bolyai University, 11 A. Janos Street, Cluj-Napoca 400028, Romania; E-Mails:

关键词: antioxidant and antimicrobial activities;    Hyssopus officinalis;    Ocimum basilicum;    Teucrium chamaedrys;    polyphenols;   

DOI  :  10.3390/molecules19055490

来源: mdpi

【 摘 要 】

This study was designed to examine the in vitro antioxidant and antimicrobial activities and to characterize the polyphenolic composition of the ethanolic extracts of Hyssopus officinalis, Ocimum basilicum and Teucrium chamaedrys. Qualitative and quantitative analysis of the major phenolic compounds were conducted using high-performance liquid chromatography coupled to mass spectrometry (HPLC-MS). The total polyphenols, caffeic acid derivatives and flavonoids content was spectrophotometrically determined. The phenolic profile showed the presence of phenolic acid derivatives (caftaric, gentisic, caffeic, p-coumaric, chlorogenic and ferulic acids), flavonoid glycosides (rutin, isoquercitrin and quercitrin) and free flavonoid aglycons (luteolin, quercetin), in different concentrations. DPPH radical scavenging assay, Trolox equivalent antioxidant capacity (TEAC) method, hemoglobin ascorbate peroxidase activity inhibition (HAPX) assay, and electron paramagnetic resonance (EPR) radicals detection were employed, revealing several aspects of the antioxidant activities of these species. The antimicrobial tests were performed using the disk diffusion assay. These extracts contained a large amount of the polyphenolic compounds (77.72, 175.57, and 243.65 mg/g, respectively), and they showed a good antioxidant activity, as witnessed by a number of methods. T. chamaedrys had a high antimicrobial activity. Besides their antioxidant activity, the antimicrobial effect of these extracts confirms the biological activities of these herbal medicinal products.

【 授权许可】

CC BY   
© 2014 by the authors; licensee MDPI, Basel, Switzerland.