Cyclodextrin-Complexed Ocimum basilicum Leaves Essential Oil Increases Fos Protein Expression in the Central Nervous System and Produce an Antihyperalgesic Effect in Animal Models for Fibromyalgia

International Journal of Molecular Sciences

Simone S. Nascimento²  Adriano A. S. Araújo⁶  Renan G. Brito²  Mairim R. Serafini⁶  Paula P. Menezes⁶  Josimari M. DeSantana⁷  Waldecy Lucca Júnior⁸  Pericles B. Alves³  Arie F. Blank⁴  Rita C. M. Oliveira⁹  Aldeidia P. Oliveira⁹  Ricardo L. C. Albuquerque-Júnior⁵  Jackson R. G. S. Almeida¹  Lucindo J. Quintans-Júnior² 

[1] Department of Pharmacy, Federal University of San Francisco Valley, Petrolina, Pernambuco 56.304-917, Brazil; E-Mail:;Laboratory of Pre-Clinical Pharmacology (LAPEC), Department of Physiology, Federal University of Sergipe, Av. Tancredo Neves, S/N, Rosa Elza, CEP: 49.000-100, São Cristóvão, Sergipe 49.100-000, Brazil; E-Mails:;Department of Chemistry, Federal University of Sergipe, São Cristóvão, Sergipe 49.100-000, Brazil; E-Mail:;Department of Agronomic Engineering, Federal University of Sergipe, São Cristóvão, Sergipe 49.100-000, Brazil; E-Mail:;Institute of Technology and Research, University Tiradentes, Aracaju, Sergipe 49.032-490, Brazil; E-Mail:;Department of Pharmacy, Federal University of Sergipe, São Cristóvão, Sergipe 49.100-000, Brazil; E-Mails:;Department of Physical Therapy, Federal University of Sergipe, Aracaju, Sergipe 49.060-108, Brazil; E-Mail:;Department of Morphology, Federal University of Sergipe, São Cristóvão, Sergipe 49.100-000, Brazil; E-Mail:;Medicinal Plants Research Center, Federal University of Piauí, Teresina, Piauí 64.049-550, Brazil; E-Mails:

关键词: Ocimum basilicum;    essential oil;    β-cyclodextrin;    pain;    fos protein;    fibromyalgia;   

DOI  :  10.3390/ijms16010547

来源: mdpi

【 摘 要 】

O. basilicum leaves produce essential oils (LEO) rich in monoterpenes. The short half-life and water insolubility are limitations for LEO medical uses. β-Cyclodextrin (β-CD) has been employed to improve the pharmacological properties of LEO. We assessed the antihyperalgesic profile of LEO, isolated or complexed in β-CD (LEO/β-CD), on an animal model for fibromyalgia. Behavioral tests: mice were treated every day with either LEO/β-CD (25, 50 or 100 mg/kg, p.o.), LEO (25 mg/kg, p.o.), tramadol (TRM 4 mg/kg, i.p.) or vehicle (saline), and 60 min after treatment behavioral parameters were assessed. Therefore, mice were evaluated for mechanical hyperalgesia (von Frey), motor coordination (Rota-rod) and muscle strength (Grip Strength Metter) in a mice fibromyalgia model. After 27 days, we evaluated the central nervous system (CNS) pathways involved in the effect induced by experimental drugs through immunofluorescence protocol to Fos protein. The differential scanning analysis (DSC), thermogravimetry/derivate thermogravimetry (TG/DTG) and infrared absorption spectroscopy (FTIR) curves indicated that the products prepared were able to incorporate the LEO efficiently. Oral treatment with LEO or LEO-βCD, at all doses tested, produced a significant reduction of mechanical hyperalgesia and we were able to significantly increase Fos protein expression. Together, our results provide evidence that LEO, isolated or complexed with β-CD, produces analgesic effects on chronic non-inflammatory pain as fibromyalgia.

【 授权许可】

CC BY   
© 2014 by the authors; licensee MDPI, Basel, Switzerland.