Radiation-Induced Changes in Serum Lipidome of Head and Neck Cancer Patients

International Journal of Molecular Sciences

Karol Jelonek⁵  Monika Pietrowska⁵  Malgorzata Ros⁵  Adam Zagdanski¹  Agnieszka Suchwalko⁴  Joanna Polanska²  Michal Marczyk²  Tomasz Rutkowski⁵  Krzysztof Skladowski⁵  Malcolm R. Clench³ 

[1] Institute of Mathematics and Computer Science, Wroclaw University of Technology, Janiszewskiego 14a, 50-370 Wroclaw, Poland; E-Mail:;Faculty of Automatic Control, Electronics and Computer Science, Silesian University of Technology, Akademicka 16, 44-100 Gliwice, Poland; E-Mails:;Biomedical Research Centre, Sheffield Hallam University, Sheffield S1 1WB, UK; E-Mail:;Institute of Biomedical Engineering and Instrumentation, Wroclaw University of Technology, Wybrzeze Wyspianskiego 27, 50-370 Wroclaw, Poland; E-Mail:;Center for Translational Research and Molecular Biology of Cancer, Maria Sklodowska-Curie Memorial Cancer Center and Institute of Oncology, Gliwice Branch, Wybrzeze Armii Krajowej 15, 44-100 Gliwice, Poland; E-Mails:

关键词: dose-volume effect;    intensity-modulated radiation therapy;    mass spectrometry;    radiation toxicity;    serum lipidome;   

DOI  :  10.3390/ijms15046609

来源: mdpi

【 摘 要 】

Cancer radiotherapy (RT) induces response of the whole patient’s body that could be detected at the blood level. We aimed to identify changes induced in serum lipidome during RT and characterize their association with doses and volumes of irradiated tissue. Sixty-six patients treated with conformal RT because of head and neck cancer were enrolled in the study. Blood samples were collected before, during and about one month after the end of RT. Lipid extracts were analyzed using MALDI-oa-ToF mass spectrometry in positive ionization mode. The major changes were observed when pre-treatment and within-treatment samples were compared. Levels of several identified phosphatidylcholines, including (PC34), (PC36) and (PC38) variants, and lysophosphatidylcholines, including (LPC16) and (LPC18) variants, were first significantly decreased and then increased in post-treatment samples. Intensities of changes were correlated with doses of radiation received by patients. Of note, such correlations were more frequent when low-to-medium doses of radiation delivered during conformal RT to large volumes of normal tissues were analyzed. Additionally, some radiation-induced changes in serum lipidome were associated with toxicity of the treatment. Obtained results indicated the involvement of choline-related signaling and potential biological importance of exposure to clinically low/medium doses of radiation in patient’s body response to radiation.

【 授权许可】

CC BY   
© 2014 by the authors; licensee MDPI, Basel, Switzerland