Xanthan Gum Removal for 1H-NMR Analysis of the Intracellular Metabolome of the Bacteria Xanthomonas axonopodis pv. citri 306

Metabolites

Vanessa R. Pegos¹  Rafael R. Canevarolo¹  Aline P. Sampaio¹  Andrea Balan¹ 

[1] Brazilian Biosciences National Laboratory, Rua Giuseppe Máximo Scolfaro, 10.000-Polo II de Alta Tecnologia, Caixa Postal 6192, CEP 13083-970, Campinas/SP, Brazil; E-Mails:

关键词: Xanthomonas;    xanthan gum removal;    ¹H-NMR;    cell extraction protocol;    metabolomics of bacteria;    metabolomics of microorganisms;   

DOI  :  10.3390/metabo4020218

来源: mdpi

【 摘 要 】

Xanthomonas is a genus of phytopathogenic bacteria, which produces a slimy, polysaccharide matrix known as xanthan gum, which involves, protects and helps the bacteria during host colonization. Although broadly used as a stabilizer and thickener in the cosmetic and food industries, xanthan gum can be a troubling artifact in molecular investigations due to its rheological properties. In particular, a cross-reaction between reference compounds and the xanthan gum could compromise metabolic quantification by NMR spectroscopy. Aiming at an efficient gum extraction protocol, for a ¹H-NMR-based metabolic profiling study of Xanthomonas, we tested four different interventions on the broadly used methanol-chloroform extraction protocol for the intracellular metabolic contents observation. Lower limits for bacterial pellet volumes for extraction were also probed, and a strategy is illustrated with an initial analysis of X. citri’s metabolism by ¹H-NMR spectroscopy.

【 授权许可】

CC BY   
© 2014 by the authors; licensee MDPI, Basel, Switzerland.