Metabolite Profiling of Diverse Rice Germplasm and Identification of Conserved Metabolic Markers of Rice Roots in Response to Long-Term Mild Salinity Stress

International Journal of Molecular Sciences

Myung Hee Nam⁵  Eunjung Bang⁴  Taek Yun Kwon²  Yuran Kim⁵  Eun Hee Kim¹  Kyungwon Cho⁵  Woong June Park³  Beom-Gi Kim²  In Sun Yoon² 

[1] Protein Structure Team, Korea Basic Science Institute, Cheongju 28119, Korea; E-Mail:;Molecular Breeding Division, National Academy of Agricultural Science, Jeonju 565-851, Korea; E-Mails:;Department of Molecular Biology, Institute of Nanosensor and Biotechnology, Dankook University, Yongin-si, Gyeonggi-do 448-701, Korea; E-Mail:;Omics System Research Team, Korea Basic Science Institute, Seoul 03759, Korea; E-Mail:;Environmental Risk and Welfare Research Team, Korea Basic Science Institute, Seoul 02855, Korea; E-Mails:

关键词: rice germplasm;    salt stress;    root;    ¹H-NMR;    metabolite markers;   

DOI  :  10.3390/ijms160921959

来源: mdpi

【 摘 要 】

The sensitivity of rice to salt stress greatly depends on growth stages, organ types and cultivars. Especially, the roots of young rice seedlings are highly salt-sensitive organs that limit plant growth, even under mild soil salinity conditions. In an attempt to identify metabolic markers of rice roots responding to salt stress, metabolite profiling was performed by ¹H-NMR spectroscopy in 38 rice genotypes that varied in biomass accumulation under long-term mild salinity condition. Multivariate statistical analysis showed separation of the control and salt-treated rice roots and rice genotypes with differential growth potential. By quantitative analyses of ¹H-NMR data, five conserved salt-responsive metabolic markers of rice roots were identified. Sucrose, allantoin and glutamate accumulated by salt stress, whereas the levels of glutamine and alanine decreased. A positive correlation of metabolite changes with growth potential and salt tolerance of rice genotypes was observed for allantoin and glutamine. Adjustment of nitrogen metabolism in rice roots is likely to be closely related to maintain the growth potential and increase the stress tolerance of rice.

【 授权许可】

CC BY   
© 2015 by the authors; licensee MDPI, Basel, Switzerland.