Quorum Sensing Activity of a Kluyvera sp. Isolated from a Malaysian Waterfall

Sensors

Nina Yusrina Muhamad Yunos¹  Wen-Si Tan¹  Nur Izzati Mohamad¹  Pui-Wan Tan¹  Tan-Guan-Sheng Adrian¹  Wai-Fong Yin¹ 

[1] Division of Genetics and Molecular Biology, Institute of Biological Sciences, Faculty of Science, University of Malaya, 50603 Kuala Lumpur, Malaysia;

关键词: cell-to-cell communication;    mass spectrometry;    triple quadrupole liquid chromatography mass spectrometry;    N-(3-oxohexanoyl)homoserine lactone;    N-3-oxo-octanoyl-L-homoserine lactone (3-oxo-C8 HSL);    Kluyvera sp;   

DOI  :  10.3390/s140508305

来源: mdpi

【 摘 要 】

In many species of bacteria, the quorum sensing mechanism is used as a unique communication system which allows them to regulate gene expression and behavior in accordance with their population density. N-Acylhomoserine lactones (AHLs) are known as diffusible autoinducer molecules involved in this communication network. This finding aimed to characterize the production of AHL of a bacterial strain ND04 isolated from a Malaysian waterfall. Strain ND04 was identified as Kluyvera sp. as confirmed by molecular analysis of its 16S ribosomal RNA gene sequence. Kluyvera sp. is closely related to the Enterobacteriaceae family. Chromobacterium violaceum CV026 was used as a biosensor to detect the production of AHL by strain ND04. High resolution triple quadrupole liquid chromatography-mass spectrometry analysis of strain ND04 showed our isolate produced two AHLs which are N-(3-oxohexanoyl)homoserine lactone (3-oxo-C6 HSL) and N-3-oxo-octanoyl-L-homoserine lactone (3-oxo-C8 HSL).

【 授权许可】

CC BY   
© 2014 by the authors; licensee MDPI, Basel, Switzerland.