Discovery of Pantoea rodasii Strain ND03 that Produces N-(3-Oxo-hexanoyl)-l-homoserine Lactone

Sensors

Nina Yusrina Muhamad Yunos¹  Wen-Si Tan¹  Nur Izzati Mohamad¹  Pui-Wan Tan¹  Tan-Guan-Sheng Adrian¹  Wai-Fong Yin¹ 

[1] Division of Genetics and Molecular Biology, Institute of Biological Sciences, Faculty of Science, University of Malaya, 50603 Kuala Lumpur, Malaysia;

关键词: cell-to-cell communication;    mass spectrometry;    triple quadrupole liquid chromatography mass spectrometry;    quorum sensing;    N-(3-oxohexanoyl) homoserine-lactone (3-oxo-C6-HSL);    Pantoea rodasii;   

DOI  :  10.3390/s140509145

来源: mdpi

【 摘 要 】

Proteobacteria use quorum sensing to regulate target gene expression in response to population density. Quorum sensing (QS) is achieved via so-called signalling molecules and the best-studied QS signalling system uses N-acyl homoserine lactones (AHLs). This study aimed to identify and characterize the production of AHLs by a bacterium ND03 isolated from a Malaysian tropical rainforest waterfall. Molecular identification showed that ND03 is a Pantoea sp. closely related to Pantoea rodasii. We used Chromobacterium violaceum CV026, an AHL biosensor for preliminary AHL production screening and then used high resolution triple quadrupole liquid chromatography-mass spectrometry, to confirm that P. rodasii strain ND03 produced N-(3-oxo-hexanoyl)-l-homoserine lactone (3-oxo-C6-HSL). To the best of our knowledge, this is the first report for such a discovery in P. rodasii strain ND03.

【 授权许可】

CC BY   
© 2014 by the authors; licensee MDPI, Basel, Switzerland.