MicroRNA-103 Promotes Colorectal Cancer by Targeting Tumor Suppressor DICER and PTEN

International Journal of Molecular Sciences

Li Geng²  Bing Sun³  Bo Gao²  Zheng Wang²  Cheng Quan²  Feng Wei¹ 

[1] Department of Hepatobiliary and Pancreas Surgery, the First Hospital, Jilin University, Changchun 130021, Jilin, China;Department of General Surgery, the Second Hospital of Jilin University, Changchun 130041, Jilin, China; E-Mails:;Department of Neurology, Changchun Central Hospital, Changchun 130041, Jilin, China; E-Mail:

关键词: miR-103;    DICER;    PTEN;    proliferation;    migration;    colorectal cancer;   

DOI  :  10.3390/ijms15058458

来源: mdpi

【 摘 要 】

MicroRNAs (miRNAs) are a class of small, noncoding RNAs that act as key regulators in various physiological and pathological processes. However, the regulatory mechanisms for miRNAs in colorectal cancer remain largely unknown. Here, we found that miR-103 is up-regulated in colorectal cancer and its overexpression is closely associated with tumor proliferation and migration. In addition, repressing the expression of miR-103 apparently inhibits colorectal cancer cell proliferation and migration in vitro and HCT-116 xenograft tumor growth in vivo. Subsequent software analysis and dual-luciferase reporter assay identified two tumor suppressor genes DICER and PTEN as direct targets of miR-103, and up-regulation of DICER and PTEN obtained similar results to that occurred in the silencing of miR-103. In addition, restoration of DICER and PTEN can inhibit miR-103-induced colorectal cancer cell proliferation and migration. Our data collectively demonstrate that miR-103 is an oncogene miRNA that promotes colorectal cancer proliferation and migration through down-regulation of the tumor suppressor genes DICER and PTEN. Thus, miR-103 may represent a new potential diagnostic and therapeutic target for colorectal cancer treatment.

【 授权许可】

CC BY   
© 2014 by the authors; licensee MDPI, Basel, Switzerland