Unusual Multiple Production of N-Acylhomoserine Lactones a by Burkholderia sp. Strain C10B Isolated from Dentine Caries

Sensors

Share Yuan Goh¹  Wen-Si Tan¹  Saad Ahmed Khan²  Hooi Pin Chew²  Noor Hayaty Abu Kasim²  Wai-Fong Yin¹ 

[1] Division of Genetics and Molecular Biology, Institute of Biological Sciences, Faculty of Science, University of Malaya, 50603 Kuala Lumpur, Malaysia; E-Mails:;Department of Restorative Dentistry, Faculty of Dentistry, University of Malaya, 50603 Kuala Lumpur, Malaysia; E-Mails:

关键词: Chromobacterium violaceum CV026;    N-hexanoyl-L-homoserine lactone (C6-HSL);    N-octanoyl-L-homoserine lactone (C8-HSL);    N-decanoyl-L-homoserine lactone (C10-HSL);    N-dodecanoyl-L-homoserine lactone (C12-HSL);    oral cavity;    dentine caries;    quorum sensing (QS);   

DOI  :  10.3390/s140508940

来源: mdpi

【 摘 要 】

Bacteria realize the ability to communicate by production of quorum sensing (QS) molecules called autoinducers, which regulate the physiological activities in their ecological niches. The oral cavity could be a potential area for the presence of QS bacteria. In this study, we report the isolation of a QS bacterial isolate C10B from dentine caries. Preliminary screening using Chromobacterium violaceum CV026 biosensor showed that isolate C10B was able to produce N-acylhomoserine lactones (AHLs). This bacterium was further identified as a member of Burkholderia, an opportunistic pathogen. The isolated Burkholderia sp. was confirmed to produce N-hexanoyl-L-homoserine lactone (C6-HSL), N-octanoyl-L-homoserine lactone (C8-HSL), N-decanoyl-L-homoserine lactone (C10-HSL) and N-dodecanoyl-L-homoserine lactone (C12-HSL).

【 授权许可】

CC BY   
© 2014 by the authors; licensee MDPI, Basel, Switzerland.