Genetic Variants in Human Leukocyte Antigen-DP Influence Both Hepatitis C Virus Persistence and Hepatitis C Virus F Protein Generation in the Chinese Han Population

International Journal of Molecular Sciences

Xiaodong Xu¹  Ming Yue⁷  Longfeng Jiang²  Xiaozhao Deng¹  Yongxiang Zhang²  Yun Zhang³  Danyan Zhu¹  Wen Xiao⁷  Zhenxian Zhou⁴  Wenjuan Yao⁵  Jing Kong⁶  Xiaojie Yu¹ 

[1]Department of Biochemistry and Molecular Biology, School of Basic Medicine, Nanjing Medical University, Nanjing 210029, China

[2] E-Mails:

[3]Department of Infectious Diseases, the First Affiliated Hospital with Nanjing Medical University, Nanjing 210029, China

[4] E-Mail:

[5]Institute of Disease Control and Prevention, Huadong Research Institute for Medicine and Biotechnics, Nanjing 210002, China

[6]Department of Clinical Laboratory, Nanjing Second Hospital, Nanjing 210003, China

[7] E-Mail:

[8]Department of Pharmacology, Nantong University Medical College, Nantong 226019, China

[9] E-Mail:

[10]School of Life Science and Chemical Engineering, Huaiyin Institute of Technology, Huaian 223003, China

[11] E-Mail:

[12]School of Life Science and Technology, China Pharmaceutical University, Nanjing 210009, China

[13] E-Mails:

关键词: F protein;    hepatitis C virus;    human leukocyte antigen-DP;    polymorphism;   

DOI  :  10.3390/ijms15069826

来源: mdpi

【 摘 要 】

Chronic hepatitis C is a serious liver disease that often results in cirrhosis or hepatocellular carcinoma. The aim of this study was to assess the association of human leukocyte antigen-DP (HLA-DP) variants with risk of chronic hepatitis C virus (HCV) or anti-F antibody generation. We selected two single nucleotide polymorphisms (SNPs) in a region including HLA-DPA1 (rs3077) and HLA-DPB1 (rs9277534) and genotyped SNPs in 702 cases and 342 healthy controls from the Chinese population using TaqMan SNP genotyping assay. Moreover, the exon 2 of the HLA-DPA1 and HLA-DPB1 genes were amplified and determined by sequencing-based typing (SBT). The results showed that rs3077 significantly increased the risk of chronic HCV infection in additive models and dominant models (odds ratio (OR) = 1.32 and 1.53). The rs3077 also contributed to decrease the risk of anti-F antibody generation in additive models and dominant models (OR = 0.46 and 0.56). Subsequent analyses revealed the risk haplotypes (DPA1*0103-DPB1*0501 and DPA1*0103-DPB1*0201) and protective haplotypes (DPA1*0202-DPB1*0501 and DPA1*0202-DPB1*0202) to chronic HCV infection. Moreover, we also found that the haplotype of DPA1*0103-DPB1*0201 and DPA1*0202-DPB1*0202 were associated with the anti-F antibody generation. Our findings show that genetic variants in HLA-DP gene are associated with chronic HCV infection and anti-F antibody generation.

【 授权许可】

CC BY   
© 2014 by the authors; licensee MDPI, Basel, Switzerland.