Metabolomic Tools for Secondary Metabolite Discovery from Marine Microbial Symbionts

Marine Drugs

Lynsey Macintyre¹  Tong Zhang¹  Christina Viegelmann¹  Ignacio Juarez Martinez¹  Cheng Cheng¹  Catherine Dowdells¹  Usama Ramadan Abdelmohsen²  Christine Gernert²  Ute Hentschel² 

[1] Strathclyde Institute of Pharmacy and Biomedical Sciences, University of Strathclyde, 161 Cathedral Street, Glasgow G4 0RE, UK; E-Mails:;Department of Botany II, Julius-von-Sachs Institute for Biological Sciences, University of Würzburg, Julius-von-Sachs-Platz 3, D-97082 Würzburg, Germany; E-Mails:

关键词: metabolomics;    dereplication;    symbiotic bacteria;    mass spectrometry;    NMR;    multivariate analysis;    metabolic profiling;   

DOI  :  10.3390/md12063416

来源: mdpi

【 摘 要 】

Marine invertebrate-associated symbiotic bacteria produce a plethora of novel secondary metabolites which may be structurally unique with interesting pharmacological properties. Selection of strains usually relies on literature searching, genetic screening and bioactivity results, often without considering the chemical novelty and abundance of secondary metabolites being produced by the microorganism until the time-consuming bioassay-guided isolation stages. To fast track the selection process, metabolomic tools were used to aid strain selection by investigating differences in the chemical profiles of 77 bacterial extracts isolated from cold water marine invertebrates from Orkney, Scotland using liquid chromatography-high resolution mass spectrometry (LC-HRMS) and nuclear magnetic resonance (NMR) spectroscopy. Following mass spectrometric analysis and dereplication using an Excel macro developed in-house, principal component analysis (PCA) was employed to differentiate the bacterial strains based on their chemical profiles. NMR ¹H and correlation spectroscopy (COSY) were also employed to obtain a chemical fingerprint of each bacterial strain and to confirm the presence of functional groups and spin systems. These results were then combined with taxonomic identification and bioassay screening data to identify three bacterial strains, namely Bacillus sp. 4117, Rhodococcus sp. ZS402 and Vibrio splendidus strain LGP32, to prioritize for scale-up based on their chemically interesting secondary metabolomes, established through dereplication and interesting bioactivities, determined from bioassay screening.

【 授权许可】

CC BY   
© 2014 by the authors; licensee MDPI, Basel, Switzerland.