Marine Drugs | |
Accurate Dereplication of Bioactive Secondary Metabolites from Marine-Derived Fungi by UHPLC-DAD-QTOFMS and a MS/HRMS Library | |
Sara Kildgaard1  Maria Mansson1  Ina Dosen1  Andreas Klitgaard1  Jens C. Frisvad1  Thomas O. Larsen1  | |
[1] Department of Systems Biology, Technical University of Denmark, Soeltofts Plads 221, Kgs. Lyngby DK-2800, Denmark; | |
关键词: marine-derived; MS/MS; dereplication; library; peptaibiotics; metabolomics; | |
DOI : 10.3390/md12063681 | |
来源: mdpi | |
【 摘 要 】
In drug discovery, reliable and fast dereplication of known compounds is essential for identification of novel bioactive compounds. Here, we show an integrated approach using ultra-high performance liquid chromatography-diode array detection-quadrupole time of flight mass spectrometry (UHPLC-DAD-QTOFMS) providing both accurate mass full-scan mass spectrometry (MS) and tandem high resolution MS (MS/HRMS) data. The methodology was demonstrated on compounds from bioactive marine-derived strains of
【 授权许可】
CC BY
© 2014 by the authors; licensee MDPI, Basel, Switzerland.
【 预 览 】
Files | Size | Format | View |
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RO202003190024518ZK.pdf | 692KB | download |