Viruses | |
Engineered RNase P Ribozymes Effectively Inhibit Human Cytomegalovirus Gene Expression and Replication | |
Zhu Yang4  Gia-Phong Vu1  Hua Qian3  Yuan-Chuan Chen1  Yu Wang2  Michael Reeves5  Ke Zen4  | |
[1] Program in Comparative Biochemistry, University of California, Berkeley, CA 94720, USA; E-Mails:;Taizhou Institute of Virology, Taizhou, Jiangsu 225300, China; E-Mail:;Department of Gynecology, People’s Hospital of Taizhou, Taizhou, Jiangsu 225300, China; E-Mail:;Institute of Virology, School of Life Sciences, Nanjing University, Nanjing, Jiangsu 210093, China; E-Mails:;School of Public Health, University of California, Berkeley, CA 94720, USA; E-Mail: | |
关键词: ribozyme; RNase P; gene targeting; cytomegalovirus; | |
DOI : 10.3390/v6062376 | |
来源: mdpi | |
【 摘 要 】
RNase P ribozyme can be engineered to be a sequence-specific gene-targeting agent with promising application in both basic research and clinical settings. By using an in vitro selection system, we have previously generated RNase P ribozyme variants that have better catalytic activity in cleaving an mRNA sequence than the wild type ribozyme. In this study, one of the variants was used to target the mRNA encoding human cytomegalovirus (HCMV) essential transcription factor immediate-early protein 2 (IE2). The variant was able to cleave IE2 mRNA
【 授权许可】
CC BY
© 2014 by the authors; licensee MDPI, Basel, Switzerland.
【 预 览 】
Files | Size | Format | View |
---|---|---|---|
RO202003190025002ZK.pdf | 790KB | download |