The Effect of Extra Virgin Olive Oil and Soybean on DNA, Cytogenicity and Some Antioxidant Enzymes in Rats

Nutrients

Thanaa A. El-Kholy⁶  Mohammad Abu Hilal⁵  Hatim Ali Al-Abbadi¹  Abdulhalim Salim Serafi³  Ahmad K. Al-Ghamdi⁴  Hanan M. Sobhy² 

[1] Experimental Surgery Unit, KFMRC (King Fahd Medical Research Center), Faculty of Medicine, King Abdulaziz University, Jeddah 21589, Saudi Arabia; E-Mail:;Pharmacology Unit, Biochemistry, Toxicology and Food deficiency Department, Animal Health Research Institute, Dokki, Gizza, Egypt; E-Mail:;Faculty of Medicine, Umm Al Qura University, Makkah 21955, Saudi Arabia; E-Mail:;Department of Medical Laboratory Technology, Faculty of Applied Medical Sciences, King Abdulaziz University, P.O. Box 80200, Jeddah 21589, Saudi Arabia; E-Mail:;School of Medicine, University of Southampton, Southampton SO16 6YD, UK; E-Mail:;Department of Clinical Nutrition, Faculty of Applied Medical Sciences, King Abdulaziz University, P.O. Box 80200, Jeddah 21589, Saudi Arabia

关键词: soybean;    olive oil;    supplements;    rats;   

DOI  :  10.3390/nu6062376

来源: mdpi

【 摘 要 】

We investigated the effect of extra virgin (EV) olive oil and genetically modified (GM) soybean on DNA, cytogenicity and some antioxidant enzymes in rodents. Forty adult male albino rats were used in this study and divided into four groups. The control group of rodents was fed basal ration only. The second group was given basal ration mixed with EV olive oil (30%). The third group was fed basal ration mixed with GM (15%), and the fourth group survived on a combination of EV olive oil, GM and the basal ration for 65 consecutive days. On day 65, blood samples were collected from each rat for antioxidant enzyme analysis. In the group fed on basal ration mixed with GM soyabean (15%), there was a significant increase in serum level of lipid peroxidation, while glutathione transferase decreased significantly. Interestingly, GM soyabean increased not only the percentage of micronucleated polychromatic erythrocytes (MPCE), but also the ratio of polychromatic erythrocytes to normochromatic erythrocytes (PEC/NEC); however, the amount of DNA and NCE were significantly decreased. Importantly, the combination of EV olive oil and GM soyabean significantly altered the tested parameters towards normal levels. This may suggest an important role for EV olive oil on rodents’ organs and warrants further investigation in humans.

【 授权许可】

CC BY   
© 2014 by the authors; licensee MDPI, Basel, Switzerland.