Cis-[Cr(C2O4)(pm)(OH2)2]+ Coordination Ion as a Specific Sensing Ion for H2O2 Detection in HT22 Cells

Molecules

Dagmara Jacewicz³  Kamila Siedlecka-Kroplewska²  Joanna Pranczk³  Dariusz Wyrzykowski³  Michał Woźniak¹ 

[1] Department of Medical Chemistry, Medical University of Gdańsk, Dębinki 1, Gdańsk 80-211, Poland; E-Mail:;Department of Histology, Medical University of Gdańsk, Dębinki 1, Gdańsk 80-211, Poland; E-Mail:;Faculty of Chemistry, University of Gdańsk, Wita Stwosza 63, Gdańsk 80-308, Poland; E-Mails:

关键词: glutamate;    HT-22 cells;    hydrogen peroxide;    kinetic mechanism;    Cr(III) complexes;    pyridoxamine;   

DOI  :  10.3390/molecules19068533

来源: mdpi

【 摘 要 】

The purpose of this study was to examine the application of the coordinated cis-[Cr(C₂O₄)(pm)(OH)₂]⁺ cation where pm denotes pyridoxamine, as a specific sensing ion for the detection of hydrogen peroxide (H₂O₂). The proposed method for H₂O₂ detection includes two key steps. The first step is based on the nonenzymatic decarboxylation of pyruvate upon reaction with H₂O₂, while the second step is based on the interaction of cis-[Cr(C₂O₄)(pm)(OH₂)₂]⁺ with the CO₂ released in the previous step. Using this method H₂O₂ generated during glutamate-induced oxidative stress was detected in HT22 hippocampal cells. The coordination ion cis-[Cr(C₂O₄)(pm)(OH₂)₂]⁺ and the spectrophotometric stopped-flow technique were applied to determine the CO₂ concentration in cell lysates, supernatants and cell-free culture medium. Prior to CO₂ assessment pyruvate was added to all samples studied. Pyruvate reacts with H₂O₂ with 1:1 stoichiometry, and consequently the amount of CO₂ released in this reaction is equivalent to the amount of H₂O₂.

【 授权许可】

CC BY   
© 2014 by the authors; licensee MDPI, Basel, Switzerland.