TRAF6 Inhibition Rescues Dexamethasone-Induced Muscle Atrophy

International Journal of Molecular Sciences

Hualin Sun¹  Yanpei Gong¹  Jiaying Qiu¹  Yanfei Chen¹  Fei Ding¹ 

[1] Jiangsu Key Laboratory of Neuroregeneration, Co-Innovation Center of Neuroregeneration, Nantong University, Nantong 226001, Jiangsu, China; E-Mails:

关键词: TRAF6;    muscle atrophy;    glucocorticoid;   

DOI  :  10.3390/ijms150611126

来源: mdpi

【 摘 要 】

Tumor necrosis factor receptor-associated factor 6 (TRAF6), a unique E3 ubiquitin ligase and adaptor protein, is involved in activation of various signaling cascades. Recent studies identify TRAF6 as one of the novel regulators of skeletal muscle atrophy. The role of TRAF6 in glucocorticoid-induced muscle atrophy, however, remains to be elucidated. In this study, we show that TRAF6 and its downstream signaling molecules, muscle atrophy F-box (MAFBx) and muscle ring finger 1 (MuRF1), were all upregulated in dexamethasone-induced atrophy of mouse C2C12 myotubes or mouse tibialis anterior (TA) muscle. To further investigate the role of TRAF6 in dexamethasone-induced muscle atrophy, TRAF6-siRNA was used to transfect cultured C2C12 myotubes or was injected into the TA muscle of mice respectively, and we note that TRAF6 knockdown attenuated dexamethasone-induced muscle atrophy in vitro and in vivo, and concomitantly decreased the expression of MuRF1 and MAFBx. Our findings suggest that a decreased expression of TRAF6 could rescue dexamethasone-induced skeletal muscle atrophy through, at least in part, regulation of the expression of MAFBx and MuRF1.

【 授权许可】

CC BY   
© 2014 by the authors; licensee MDPI, Basel, Switzerland.