Inhibition of TRPM7 Channels Reduces Degranulation and Release of Cytokines in Rat Bone Marrow-Derived Mast Cells

International Journal of Molecular Sciences

Linjie Huang³  Ngai-Mui Ng²  Ming Chen³  Xiaoling Lin³  Tiantian Tang³  Huihua Cheng³  Cheng Yang¹ 

[1] Department of Respiratory Medicine, Mei Zhou People’s Hospital, Meizhou Affiliated Hospital of Sun Yat-sen University, Meizhou 514031, China; E-Mail:;Clinical Department of School of Medicine, Shenzhen University, Shenzhen 518060, China; E-Mail:;Department of Respiratory Medicine, Sun Yat-sen Memorial Hospital, Institute for Respiratory Disease of Sun Yat-sen University, Sun Yat-sen University, Guangzhou 510120, China; E-Mails:

关键词: rat;    asthma;    bone marrow-derived mast cells (BMMCs);    transient receptor potential melastatin 7 (TRPM7);    2-aminoethoxydiphenyl borate (2-APB);    shRNA;    cytokines;   

DOI  :  10.3390/ijms150711817

来源: mdpi

【 摘 要 】

Background: mast cells play an important role in airway inflammation in asthma. The transient receptor potential melastatin-like 7 (TRPM7) channel is expressed in primary human lung mast cells and plays a critical role for cell survival. This study aimed to investigate the role of TRPM7 on degranulation and release of cytokines in rat bone marrow-derived mast cells (BMMCs). Methods: the expression levels of TRPM7 were observed by immunocytochemistry and RT-PCR between normal and asthmatic rat BMMCs. TRPM7-specific shRNA and 2-aminoethoxydiphenyl borate (2-APB) and specific shTRPM7 were used to inhibit the function of TRPM7. Degranulation levels were analyzed by beta-hexosaminidase assay. Histamine, TNF-α, IL-6 and IL-13 levels were measured by ELISA. Results: the expression of TRPM7 was significantly higher in asthmatic rat BMMCs than in the normal control group. After application of 2-APB and down-regulation of TRPM7, the beta-hexosaminidase activity and secretion of histamine, IL-6, IL-13 and TNF-α were significantly decreased in the asthmatic group compared to the control group. Conclusion: this study indicates that TRPM7 channels may be involved in the process of degranulation and release of cytokines in rat bone marrow-derived mast cells.

【 授权许可】

CC BY   
© 2014 by the authors; licensee MDPI, Basel, Switzerland.