Mulberry Fruit Extract Protects Pancreatic β-Cells against Hydrogen Peroxide-Induced Apoptosis via Antioxidative Activity

Molecules

Jong Seok Lee²  Young Rae Kim²  Jun Myoung Park²  Suk-Jin Ha²  Young Eon Kim¹  Nam In Baek³ 

[1] Korea Food Research Institute, Seongnam 463746, Korea; E-Mail:;Department of Bioengineering and Technology, Kangwon National University, Chuncheon 200701, Korea; E-Mails:;Department of Oriental Medicinal Materials and Processing, Kyung Hee University, Youngin 446701, Korea; E-Mail:

关键词: mulberry;    oxidative stress;    apoptosis;    diabetes;    pancreatic β-cells;   

DOI  :  10.3390/molecules19078904

来源: mdpi

【 摘 要 】

Among the many environmental stresses, excessive production of reactive oxygen species (ROS) and the ensuring oxidative stress are known to cause significant cellular damage. This has clinical implications in the onset of type 1 diabetes, which is triggered by the destruction of pancreatic β-cells and is associated with oxidative stress. In this study, we investigated the protective and antioxidative effects of mulberry extract (ME) in insulin-producing pancreatic β-cells. We found that ME protects pancreatic β-cells against hydrogen peroxide (H₂O₂)-induced oxidative stress and the associated apoptotic cell death. ME treatment significantly reduced the levels of H₂O₂-induced 2-diphenyl-1-picrylhydrazyl (DPPH) radicals, and lipid peroxidation and intracellular ROS accumulation. In addition, ME inhibited DNA condensation and/or fragmentation induced by H₂O₂. These results suggest that ME protects pancreatic β-cells against hydrogen peroxide-induced oxidative stress.

【 授权许可】

CC BY   
© 2014 by the authors; licensee MDPI, Basel, Switzerland.