Protective Effects of Baccharis dracunculifolia Leaves Extract against Carbon Tetrachloride- and Acetaminophen-Induced Hepatotoxicity in Experimental Animals

Molecules

Túlio P. Rezende⁴  José Otávio do A. Corrສ³  Beatriz J. V. Aarestrup¹  Fernando M. Aarestrup¹  Orlando V. de Sousa² 

[1]Laboratório de Imunopatologia e Patologia Experimental, Universidade Federal de Juiz de Fora, Rua José Lourenço Kelmer, s/n – Campus Universitário, Bairro São Pedro, CEP36036-900, Juiz de Fora, MG, Brazil

[2]Laboratório de Farmacologia de Produtos Naturais, Universidade Federal de Juiz de Fora, Rua José Lourenço Kelmer, s/n – Campus Universitário, Bairro São Pedro, CEP36036-900, Juiz de Fora, MG, Brazil

[3]Laboratório de Bioatividade Celular e Molecular, Universidade Federal de Juiz de Fora, Rua José Lourenço Kelmer, s/n – Campus Universitário, Bairro São Pedro, CEP36036-900, Juiz de Fora, MG, Brazil

[4]Núcleo de Identificação e Pesquisa em Princípios Ativos Naturais - NIPPAN, Faculdade de Farmácia, Universidade Federal de Juiz de Fora, Rua José Lourenço Kelmer, s/n – Campus Universitário, Bairro São Pedro, CEP36036-900, Juiz de Fora, MG, Brazil

关键词: Baccharis dracunculifolia;    Brazilian green propolis;    acetaminophen;    carbon tetrachloride;    hepatoprotective;   

DOI  :  10.3390/molecules19079257

来源: mdpi

【 摘 要 】

In this work we investigated the in vivo protective effects of Baccharis dracunculifolia leaves extract (BdE) against carbon tetrachloride (CCl₄)- and acetaminophen (APAP)-induced hepatotoxicity. Total phenolic content, total flavonoid content, antioxidant DPPH radical scavenging activity, and HPLC analysis were performed. Our results showed that pretreatment with BdE significantly reduced the damage caused by CCl₄ and APAP on the serum markers of hepatic injury, AST, ALT, and ALP. Results were confirmed by histopathological analysis. Phytochemical analysis, performed by HPLC, showed that BdE was rich in p-coumaric acid derivatives, caffeoylquinic acids and flavonoids. BdE also showed DPPH antioxidant activity (EC₅₀ of 15.75 ± 0.43 μg/mL), and high total phenolic (142.90 ± 0.77 mg GAE/g) and flavonoid (51.47 ± 0.60 mg RE/g) contents. This study indicated that B. dracunculifolia leaves extract has relevant in vivo hepatoprotective properties.

【 授权许可】

CC BY   
© 2014 by the authors; licensee MDPI, Basel, Switzerland.